7/21/2023 0 Comments 600 markly![]() 2014), and this publication contained an assigned 2D 1H─ 15N HSQC NMR spectrum of this domain. ![]() X-ray crystal structures of this region (PDB IDs 4QB0 and 4QAZ) were solved by the Derewenda group ( Dziubańska et al. The function of the terminal domain of eNP (eNP-CTD), usually defined as residues 641‒739, is unknown, but it is thought to engage in protein-protein interactions. In these capacities, eNP interacts with Ebola proteins L, VP35, VP24, and VP30 as well as with genomic RNA ( Messaoudi et al. We targeted the critical Ebola nucleoprotein (eNP), which has roles in genome replication, packaging, and transcription as well as viral structure. Of the seven encoded proteins, only the RNA replicase/polymerase “L” protein possesses enzymatic activity therefore, disruption of protein-protein interactions may be a viable approach to drug development. The Ebola virus genome consists of approximately 19 kb of non-segmented, single-stranded, negative-sense RNA. We found that the 600‒739 domain consists of separate regions that are largely disordered and ordered. This work supports and extends the previous X-ray crystal structures and NMR studies of residues 641‒739. In the course of this effort we assigned the backbone 1H, 15N, and 13C resonances of residues 600‒739, the region that contains the critical eVP30 binding region 600‒615 targeted by host factors, and used the assigned chemical shifts to predict secondary structural features and peptide dynamics. We carried out a screening campaign to identify small, drug-like compounds that bind to the C-terminal region of the multifunctional Ebola nucleoprotein (eNP) with the objective of discovering ones that disrupt its binding to other Ebola proteins or to the single-stranded RNA genome. Because only one of the seven proteins encoded by the viral genome possesses enzymatic activity, disruption of protein-protein interactions is a promising route for antiviral drug development. and by the time that happens, the return will most likely time out.Ebola viral infections have resulted in several deadly epidemics in recent years in West and Central Africa. ![]() that is if the forwarder actually provides that service. If a buyer has a problem with the item after it has been forwarded, the buyer needs to work with the forwarder to get the item back to them and then returned to the seller. If an item is not delivered from the forwarder, the buyer needs to deal with the forwarder.įor Item Not as Described, the seller is only responsible to pay return shipping from the original address on the order. The responsibility of the seller for an Item Not Received case ends at Delivery to the address on the order (the freight forwarder). The item was sent to another address after the original delivery, or buyer uses a freight forwarder (exceptions which are still eligible for coverage include: eBay shipping programs such as the Global Shipping Program and eBay international standard delivery) The MBG does not cover items that have been reshipped after original delivery. The seller has to deal directly through E-bay with the buyer, not the anonymous person at the transport company. Thank you for your "delivery responsibility" of the seller, most certainly does not end at the door of the transport company. Sellers can dispute these returns but again that take time and the seller will generally lose the return case. The time it takes to track an item down is almost impossible. Some over seas buyers have been known to return empty boxes to random addresses in the same zip code as seller. Sellers can dispute these returns but that takes time and the seller generally loses. E-bay always decides in favor of the buyer for returns. As you know, when an out of country buyer purchases through Pay Pal they have 180 days to return. The risk of sending items out of the country and not having them returned is too high. This has nothing to do with discrimination. I sell only in the US and my listings state "US only". ![]() Why start with an impression that is false? When you purchase an item and do not disclose ALL of the information a seller needs, #1 they are going to become suspicious and #2 are going to take steps to protect themselves. Why does a buyer not state from the beginning that the item is being shipped overseas through a transport logistic company, such as ? Why do some overseas buyers addresses not reflect the transport logistic company? For example, they should read, John Doe, Meest.US, 600 Markely St., Reading, NJ. First of all, instead of claiming "discrimination", you need to be honest with sellers.
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